![]() The preferential binding of 14-3-3 to phosphorylated C-terminal sequences, mode III, provides a means of regulated binding and considerably expands the substrate repertoire of 14-3-3 interaction partners. Signaling 14-3-3 protein signaling by recognizing phosphorylated peptides plays a critical role in a variety of biological processes, including oncogenesis. Diversity and functional significance of C-terminal sequences have been appreciated from studies of PDZ and PEX domains. However, structural information, a crucial descriptor of antibody binding capability, is not collected in Ig-seq protocols. Moreover, the antigen-binding sites of many antibodies can cross-react with a variety of related but different antigenic determinants, making the antibody. Next-generation sequencing of immunoglobulin gene repertoires (Ig-seq) allows the investigation of large-scale antibody dynamics at a sequence level. They tend to be exposed in folded three dimensional structures. Even in the absence of antigen stimulation, a human can probably make more than 10 12 different antibody moleculesits preimmune antibody repertoire. The preferential binding of 14-3-3 to phosphorylated C-terminal sequences, mode III, provides a means of regulated binding and considerably expands the substrate repertoire of 14-3-3 interaction partners.ĪB - Amino and carboxyl termini are unique positions in a polypeptide. Diversity and functional significance of C-terminal sequences have been appreciated from studies of PDZ and PEX domains. Reinherz, Definition of MHC supertypes through clustering of MHC peptide-binding repertoires, Methods in Molecular Biology, vol. ![]() They tend to be exposed in folded three dimensional structures. Phenomenon in which the binding of one ligand molecule to a target molecule promotes the binding of successive ligand molecules. N2 - Amino and carboxyl termini are unique positions in a polypeptide. In molecular immunology, the assortment of immunoglobulins produced by an animal this assortment changes ( repertoire shift) as the animal develops immunity following a challenge. GM70959), an Established Investigator Award (M.L.) and a pre-doctoral training award (B.C.) from the American Heart Association. This work is supported by grants from the National Institutes of Health (to M.L. David Klein and members of the Li laboratory for valuable discussion and comments on this manuscript. T2 - An expanded repertoire and function of 14-3-3 proteins
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